2012
Arias, Dulce M; Albarrán-Lara, Ana L; González-Rodríguez, Antonio; Peñaloza-Ramírez, Juan; Dorado, Oscar; Leyva, Esaú
Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia mexicana (Brassicales: Caricaceae) at a local scale in Mexico report
2012.
Resumen | Etiquetas: Bayesian methods, Caricaceae, Genetic diversity, Jacaratia mexicana, population structure, RAPDs
@report{Arias2012,
title = {Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia mexicana (Brassicales: Caricaceae) at a local scale in Mexico},
author = {Dulce M Arias and Ana L Albarrán-Lara and Antonio González-Rodríguez and Juan Peñaloza-Ramírez and Oscar Dorado and Esaú Leyva},
year = {2012},
date = {2012-01-01},
journal = {Rev. Biol. Trop. (Int. J. Trop. Biol. ISSN},
volume = {60},
issue = {1},
pages = {1-10},
abstract = {The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon's index. An AMOvA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893},
keywords = {Bayesian methods, Caricaceae, Genetic diversity, Jacaratia mexicana, population structure, RAPDs},
pubstate = {published},
tppubtype = {report}
}
The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon's index. An AMOvA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893